Topography and refractometry of sperm cells using spatial light interference microscopy
نویسندگان
چکیده
منابع مشابه
Topography and refractometry of nanostructures using spatial light interference microscopy.
Spatial light interference microscopy (SLIM) is a novel method developed in our laboratory that provides quantitative phase images of transparent structures with a 0.3 nm spatial and 0.03 nm temporal accuracy owing to the white light illumination and its common path interferometric geometry. We exploit these features and demonstrate SLIM's ability to perform topography at a single atomic layer ...
متن کاملTopography and refractometry of nanostructures using spatial light interference microscopy (SLIM)
Spatial Light Interference Microscopy (SLIM) is a novel method developed in our laboratory that provides quantitative phase images of transparent structures with 0.3 nm spatial and 0.03 nm temporal accuracy owing to the white light illumination and its common path interferometric geometry. We exploit these features and demonstrate SLIM's ability to perform topography at a single atomic layer in...
متن کاملInstantaneous Spatial Light Interference Microscopy.
We present Instantaneous Spatial Light Interference Microscopy (iSLIM) as a new quantitative phase method that combines the benefits of white light illumination in Zernike's phase contrast microscopy and phase stability associated diffraction phase microscopy. iSLIM is implemented as an add-on module to a commercial phase contrast microscope, and enables new features to quantitative phase imagi...
متن کاملCardiomyocyte Imaging Using Real-Time Spatial Light Interference Microscopy (SLIM)
Spatial light interference microscopy (SLIM) is a highly sensitive quantitative phase imaging method, which is capable of unprecedented structure studies in biology and beyond. In addition to the π/2 shift introduced in phase contrast between the scattered and unscattered light from the sample, 4 phase shifts are generated in SLIM, by increments of π/2 using a reflective liquid crystal phase mo...
متن کاملSuper-resolved spatial light interference microscopy.
We report a scheme to achieve resolution beyond the diffraction limit in spatial light interference microscopy (SLIM). By adding a grating to the optical path, the structured illumination technique can be used to improve the resolution by a factor of 2. We show that a direct application of the structured illumination technique, however, has proved to be unsuccessful. Through two crucial modific...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2018
ISSN: 1083-3668
DOI: 10.1117/1.jbo.23.2.025003